Analyses of bile from gallbladders of fish (Arius platystomus,Arius tenuispinis,Pomadasys commersonni and Kishinoella tonggol)

Bile from gallbladders of arius platystomus (singhara),arius tenuispinis (khagga),pomadasys commersonni (holoola) and kishinoella tonggol (dawan) were derivatised and analysed by gc-ms for identification of bile acids and bile alcohols. cholic acid and chenodeoxycholic acid were found as major bile acids in arius platystomus,arius tenuispinis and pomadasys commersonni. other bile acids identified in arius platystomus were allochenodeoxycholic acid,allodeoxycholic acid,3α,7α,12α-trihydroxy-24-methyl-5β-cholestane-26-oic acid,and 3α,7α,12α,24-tetrahydroxy-5α-cholestane-26-oic acid. cholesterol was found as major bile alcohol in arius platystomus,arius tenuispinis and pomadasys commersonni. cholic acid was the major bile acid identified in the bile of kishinoella tonggol while other bile acids included 3α,7α,12α-tridydroxy-5α-cholestanoic acid and 3α,7α,12α-tridydroxy-5β-cholestanoic acid. bile alcohol 5β-cyprinol was present in significant amounts with 5β-cholestane-3α,7α,12α,24ζ-tetrol being the other contributors in the bile of kishinoella tonggol.