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Evaluation of putative cytotoxic activity of crude extracts from Onopordum acanthium leaves and Spartium junceum flowers against the U-373 glioblastoma cell line
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نویسنده
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abusamra y.a.-k. ,scuruchi m. ,habibatni s. ,maammeri z. ,benayache s. ,d'ascola a. ,avenoso a. ,campo g.m. ,spina e.
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منبع
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pakistan journal of pharmaceutical sciences - 2015 - دوره : 28 - شماره : 4 - صفحه:1225 -1232
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چکیده
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Crude hydromethanolic (80% methanol) extracts produced by maceration of onopordum acanthium leaves and spartium junceum flowers were tested for cytotoxic effects against glioblastoma u-373 tumour cells. onopordum acanthium extract was found to be ~5 times more cytotoxic than spartium junceum (ic50 values of 309 and 1602μg/ml,respectively). similar to most chemotherapeutic agents killing through the intrinsic pathway,onopordum killed the cells via apoptosis,which was confirmed by the activation of caspase-3. spartium exerted its weak cytotoxic effect,presumably by a caspase-independent,non-apoptotic form of necrotic-like programmed cell death. onopordum acanthium is considered a promising plant for the researchers investigating putative biological activities,particularly antitumour and immune-related activity.
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کلیدواژه
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Apoptosis; Cytotoxicity; Glioblastoma U-373; Onopordum acanthium; Spartium junceum
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آدرس
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deptt. of clinical and experimental medicine,policlinico universitario,school of medicine,university of messina, Italy, deptt. of biomedical sciences and morphological and functional images,policlinico universitario,school of medicine,university of messina, Italy, laboratory of development of natural resources and synthesis of bioactive substances,mentouri university,constantine,algeria,laboratory of pharmacology and toxicology,department of veterinary sciences,mentouri university, Algeria, laboratory of pharmacology and toxicology,department of veterinary sciences,mentouri university, Algeria, laboratory of development of natural resources and synthesis of bioactive substances,mentouri university, Algeria, deptt. of biomedical sciences and morphological and functional images,policlinico universitario,school of medicine,university of messina, Italy, deptt. of biomedical sciences and morphological and functional images,policlinico universitario,school of medicine,university of messina, Italy, deptt. of biomedical sciences and morphological and functional images,policlinico universitario,school of medicine,university of messina, Italy, deptt. of clinical and experimental medicine,policlinico universitario,school of medicine,university of messina, Italy
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Authors
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