Densitometric validation and analysis of biomarker β-amyrin in different Acacia species (leaves) grown in Kingdom of Saudi Arabia by high performance thin-layer chromatography

Biomarker β-amyrin was analyzed in the leaves of four different acacia species (a. salicina,a. loreta,a. hamulosa and a. tortilis) grown in kingdom of saudi arabia by a validated hptlc method. the chromatography was performed on glass-backed silica gel 60 f254 hptlc plates using solvents toluene: methanol (9:1,v/v) as mobile phase. the developed tlc plate was derivatized with anisaldehyde and scanned at 520 nm. a sharp peak of β-amyrin was found at rf=0.58±0.01. the r2 and the linear regression equation for β-amyrin was found to be 0.991 and 19.913x+107.803,respectively in the concentration range of 100-800 ng. the percentage of β-amyrin was found to be maximum 2.70% w/w in a. tortilis,1.85% w/w in a. loreta and 1.80% w/w in a. hamulosa while it was totally absent in a. salicina. this study conceives maiden reporting of quantification of β-amyrin in four different species of acacia by validated hptlc method. the developed method for the analysis of β-amyrin was proved to be reproducible by statistical analysis hence it can be employed for further analysis of β-amyrin in plasma,other biological fluids and in finished products available in the market.