Plasmid borne BAC-IB17: Localization of a potential antibacterial positive marker (Bac+) encoded broad inhibitory spectrum bacteriocin

Production of antimicrobial compounds is considered as ubiquitous anti-competitor strategy in bacterial ecosystem. bacteriocins are heterogeneous; highly specific and efficient anti-competitor agents and the gene responsible for the production of bacteriocins mostly exist in an autosomal state and associated with plasmids. bac-ib17 is a broad spectrum bacteriocin and its production was observed at different stages of the growth cycle from bacillus subtilis kibge-ib17. growth kinetics of b. subtilis kibge-ib17 along with the production of bac-ib17 showed that it exhibited secondary metabolite kinetics. plasmid curing technique revealed that the gene responsible for the bacteriocinogenecity in b. subtilis kibge-ib17 was located on the plasmid of the bacterium. overlay method also demonstrated the plasmid-mediated bacteriocinogenesis of the isolated colonies. with the advancement in genomics and proteomics,the plasmid borne bac-ib17 can play a significant role in the transfer of bacteriocinogenic factor to other incompetent cells and also in the maintenance of plasmid in bacterial population.