Determination of α-glucosidase inhibitory activity from selected Fabaceae plants

Nineteen plants from fabaceae family,which were used in thai traditional medicine for treatment of diabetes,were determined of α-glucosidase inhibitory activity via enzymatic reaction. in this reaction,α-glucosidase was used as enzyme,which reacted with the substrate,p-nitrophenol-d-glucopyranoside (pnpg). after that the product,p-nitro phenol (pnp) will be occurred and observed the yellow colour at 405 nm. in this study,acarbose was used as positive standard which,inhibited this enzyme with ic50 as 331±4.73 μg/ml. caesalpinia pulcherrima leaves showed the highest activity with ic50 as 436.97±9.44 μg/ml. furthermore,bauhinia malabarica leaves presented moderately activity with ic50 as 745.08±11.15 μg/ml. however,the other plants showed mild to none activity of α-glucosidase inhibition. accordingly,this study can support anti-diabetes of these plants in traditional medicine and it will be the database of the biological activity of fabaceae plant.