Enumeration of CD4+CD25+ T regulatory cells in Type-II diabetes retinopathy

Diabetes mellitus (dm) is a health concern because it leads to complications such as retinopathy. pakistan has 6.9 million dm affected people that will be doubled by 2025. a study was designed to enumerate cd4+cd25+treg cells in pakistani type 2 diabetes mellitus (t2dm) patients. it was a cross-sectional case-control study that included 212 subjects. the subjects having diabetic retinopathy were labeled as group-i (30 healthy volunteers without diabetes), group-ii (30 t2dm patients without retinopathy) and group-iii (152 t2dm patients with retinopathy). the percentage of cd4+cd25+ treg cells was determined by flowcytometry. comparison of cd4+cd25+t cells among different groups was not significant and higher percentage of treg cells was observed in group-ii (3.07%) compared to group-iii (2.88%). age, gender and duration of diabetes may contribute while percentage of cd4+cd25+t cells and treg cells were not associated with the development of dr in t2dm.