Antitumor effects of a polypeptide isolated from Tegillarca granosa linnaeus and the related molecular mechanism

This study is to investigate the anticancer effects and mechanisms of tegillarca granosa linnaeus-1 (tg-1) on renal carcinoma os-rc-2 cells in vitro. the proliferation of os-rc-2 cells was evaluated under various concentrations of tg-1 using mtt assay. the apoptosis of os-rc-2 cells was analyzed using acridine orange/ethidium bromide staining. and the cell cycle distribution of os-rc-2 cells was detected by flow cytometry. in addition, the expression level of ki67 mrna was examined by rt-pcr and level of casepase-3 was examined by western blot analysis. tg-1 incubation significantly inhibited the proliferation of renal carcinoma os-rc-2 cells and arrested cells at g0/g1 phase (p <0.05). and tg-1 also significantly inhibited the expression of ki67 mrna (p<0.05). additionally, tg- 1 significantly promoted apoptosis and the expression of caspase-3 in cells (p<0.05). moreover, the optimal effects of tg-1 was achieved at the concentration of 100 mg/l the results indicate that tg-1 has antitumor effects on renal carcinoma os-rc-2 cells and that the underlying mechanisms may be acted through inhibiting proliferation and ki67 mrna expression, and promoting apoptosis and caspase-3 expression.