Determination of kaempferol and quercetin in Xindakang tablet by β-cyclodextrin modified micellar electrokinetic capillary

A method was proposed to determine kaempferol and quercetin in hippophae rhamnoides l medicinal preparation xindakang tablet by β-cyclodextrin modified micellar electrokinetic capillary chromatography. under the optimized conditions: buffer solution of 20 mmol/l na2b4o7-kh2po4 (ph 9.0)-20mmol/l sds-6mmol/l β-cd-5%(v/v) mecn, applied voltage of 16 kv and injection time of 8s, the two analytes were separated well within 10 minutes. the calibration was linear in the 0.02-0.80 and 0.02-0.70 mg/ml range for kaempferol and quercetin, respectively. the reproducibility based on migration time and peak height were 0.47% and1.87% for kaempferol, 0.55% and 2.02% for quercetin. the detection limits based on three times noise were 0.010 mg/ml and 0.008 mg/ml for kaempferol and quercetin, respectively. the developed method was utilized to analyze real samples and running recovery experiments with satisfactory results.