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Cytotoxic and antimicrobial activities of Emex spinosa (L.) Campd. extract
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نویسنده
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Donia Abd El Raheim Mohammed ,Soliman Gamal Abd El Hakim ,El Sakhawy Mohamed Abd El Monem ,Yusufoglu Hasan ,Ahmed Zaghloul Mohamed
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منبع
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pakistan journal of pharmaceutical sciences - 2014 - دوره : 27 - شماره : 2 - صفحه:351 -356
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چکیده
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The current research was designed to evaluate the phytochemical contents, cytotoxic and antimicrobial activity of emex spinosa extracts. the different plant extracts and aloe-emodin glucoside were screened using the colorimetric mtt method (3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyl- tetrazolium bromide) assay to test their in vitro cytotoxic activity against hepg2, mcf-7, caco-2 and hct. the clinically used anticancer drug doxorubicin was used as standard for comparative purposes. anthraquinones (aloe-emodin-o-glucoside, emodin and nataloin (1, 2, 8-trihydroxy, 6-methyl, 10-anthrone-c-glucoside) together with β-sistosterol and β-sitisterol-o-β-d-glucoside were isolated from emex spinosa. aloe-emodin glucoside together with four fractions from this plant were evaluated for their anticancer and antimicrobial activities. aloe-emodin glucoside showed anticancer activity against hct, hepg-2, mcf-7 and caco-2 cell lines. the total ethanol extract of e. spinosa and diethyl ether, chloroform, ethyl acetate and butanol fractions shown antibacterial activity against staphylococcus aureus, streptococcus pyogenes and bacillus subtilis.
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کلیدواژه
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The current research was designed to evaluate the phytochemical contents ,cytotoxic and antimicrobialactivity of Emex spinosa extracts. The different plant extracts and Aloe-emodin glucoside were screened using thecolorimetric MTT method (3-(4 ,5-dimethylthiazo-2-yl)-2 ,5-diphenyl- tetrazolium bromide) assay to test their in vitrocytotoxic activity against HepG2 ,MCF-7 ,Caco-2 and HCT. The clinically used anticancer drug doxorubicin was used asstandard for comparative purposes. Anthraquinones (Aloe-emodin-O-glucoside ,Emodin and nataloin (1 ,8-trihydroxy ,6-methyl ,10-anthrone-C-glucoside) together with β-sistosterol and β-sitisterol-O-β-D-glucoside were isolated fromEmex spinosa. Aloe-emodin glucoside together with four fractions from this plant were evaluated for their anticancer andantimicrobial activities. Aloe-emodin glucoside showed anticancer activity against HCT ,HepG-2 ,MCF-7 and Caco-2cell lines. The total ethanol extract of E. spinosa and diethyl ether ,chloroform ,ethyl acetate and butanol fractions shownantibacterial activity against Staphylococcus aureus ,Streptococcus pyogenes and Bacillus subtilis.
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آدرس
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Salman Bin Abdulaziz University, College of Pharmacy, Pharmacognosy Department, Saudi Arabia. Desert Research Center, Medicinal and Aromatic Plants Department, Egypt, Salman Bin Abdulaziz University, College of Pharmacy, Pharmacology Department, Saudi Arabia. Cairo University, College of Veterinary Medicine, Pharmacology Department, Egypt, Salman Bin Abdulaziz University, College of Medical and Applied Sciences, Saudi Arabia, Salman Bin Abdulaziz University, College of Pharmacy, Pharmacognosy Department, Saudi Arabia, Salman Bin Abdulaziz University, College of Pharmacy, Pharmacognosy Department, Saudi Arabia. Mansoura University, College of Pharmacy, Pharmacognosy Department, Egypt
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Authors
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