Cytotoxicity evaluation of vancomycin and its complex with betacyclodextrin on human glial cell line

The possible cytotoxic effects of vancomycin and its complex with beta-cyclodextrin (β-cd) on human glial cell line (crl 8621) were studied accordingly by means of mts assay. the cultured cells were incubated with various concentrations of vancomycin, β-cd as well as β-cd/vancomycin complex ranging from 4.69 to 300 ug/ml. a linear dose-dependency cytotoxicity followed by hermetic-like biphasic dose-dependence was observed after incubation period of 72 hours. in general, significant increase (p<0.001) of cell proliferation was observed at lower concentrations: <18.75 μg/ml for cells treated with β-cd and their complex while < 9.38 μg/ml for cells treated with vancomycin. in contrary, regardless of the treatments given, significant (p<0.001) reduce in cell survival was found at higher concentrations >150 μg/ml. in particular, 50 % inhibitory in vitro was achieved at the concentrations of 115.95 μg/ml (for β-cd), 116.48 μg/ml (for vancomycin) and 115.44 μg/ml (for β-cd/vancomycin complex).