Pharmacological and biological evaluation of extracts from Gratiola officinalis L. (Scrophulariaceae)

The crude extract of gratiola officinalis and its n-hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions were subjected to biological (brine shrimp bioassay, insecticidal and phytotoxicity/cytotoxic) and neuropharmacological (head dip, open field forced swimming test, sodium pentothal induced sleep) activities. results obtained in this study indicated that at high concentration dose (1000μg/ml), all test samples showed 60-95% phytotoxicity. in crude extract, n-butanol and aqueous fractions produced more than 85% phytotoxicity. while low concentration (10μg/ml) dose showed 25-28% phytotoxicity in all test samples. the crude extract was devoid of any effect against the growth of callosbruchus analis and tribolium castaneum and caused 10 mortality of rhyzopertha dominica. n-hexane, chloroform, ethylacetate, n-butanol and aqueous fractions caused 50, 30, 40, 10 and 20% mortality respectively of c. analis where as chloroform, ethyl-acetate, aqueous and crude extract, n-hexane, ethyl-acetate fractions also caused low mortality (10%) of tribolium castaneum and rhyzopertha dominica respectively. in cytotoxic assay at 1000μg/ml concentration, n-butanol fraction produced 36.7% and the crude extract produced 13.3% mortality of brine shrimp, its aqueous fraction was inactive at all concentrations. the results of head dip, open field, mobility time and pentothal na induced sleep indicated that crude extract, n-butanol and ethylacetate fractions of g. officinalis had mild sedative effect. however aqueous fraction was found to produce a significant decrease in motor activities and potentiated the duration of sleep.