DEVELOPMENT AND IMPLICATION OF A CAPILLARY ELECTROPHORESIS METHODOLOGY FOR CIPROFLOXACIN, PARACETAMOL AND DICLOFENAC SODIUM IN PHARMACEUTICAL FORMULATIONS AND SIMULTANEOUSLY IN HUMAN URINE SAMPLES

This work studies the development of a simple and fairly rapid methodology for simultaneous determination/separation of three frequently co-administered drugs; ciprofloxacin (cip), paracetamol (pct) and diclofenac sodium (dic) using capillary electrophoresis (ce) with uv detection at 260 nm. separation was achieved in only 6.5 min with a simple buffer of sodium tetraborate (50 mm) at ph 9.0. the parameters affecting the separation and detection were optimized. the calibration curves were linear in the range of 5-500 μg/ml for cip, 5-250 μg/ml for pct and 1-125 μg/ml for dic sodium under the optimized conditions. the lower limit of detection (lod) was found to be 1 μg/ml for cip & pct and 0.5 μg/ml for dic. the method was successfully used for the analysis of drugs in commercial pharmaceutical formulations and simultaneously from patient’s urine sample with rsd 0.5-2.4%. results obtained with ce method are compared with standard hplc procedure and were found in good agreement.