Anti-inflammatory effect of methanolic extract of Conyzacanadensis in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophage cells

The aim of this study was to investigate the potential anti-inflammatory effect of conyzacanadeusis methanol extract (cme) using a cell model of raw264.7 murine macrophage cell stimulated with lipopolysaccharide (lps)(1μg/ml). co-treatment with different concentrations (10,50 and 100μg/ml) of cme was concentrationdependently reduced the lps-induced generation of prostaglandin e2 (pge2),nitric oxide (no) tumor necrosis factor-α (tnf-α),interleukin (il)-1β and il-6. in addition,cme also reduced the mrna expressions of cyclooxygenase-2 (cox-2),inducible nitric oxide (inos),tnf-α,il-1β and il-6 in lps-stimulated raw264.7 cells. these results suggested that cme showed an anti-inflammatory activity through reduced the mrna expression of cox-2,inos,tnf-α,il-1β and il-6 and also decreased the productions of pge2,no,tnf-α,il-1β and il-6in lps-stimulated raw264.7 cells.