Evaluation of in vitro urease and lipoxygenase inhibition activity of weight reducing tablets

Enzyme inhibition is a significant part of research in pharmaceutical field in view of the fact that these studies have directed to the innovations of drugs having remarkable performance in diverse physiological conditions. the present study was aimed to assess urease and lipoxygenase inhibitory activity of weight reducing tablets. for evaluating the urease activity indophenol method was employed using thiourea as the model urease inhibitor. the lipoxygenase inhibition was evaluated by measuring the hydroperoxides produced in lipoxygenation reaction using a purified lipoxygenase with lionoleic acid as substrate. when formulation of the weight reducing tablets was compared at various concentrations (50,100 and 500µg/ml). the antiurease activity and lipoxygenase inhibition activity increased in a dose dependent manner. the formulations under test have an excellent antiurease and lipoxygenase inhibition potential and prospective to be used in the cure of a variety of complications associated with the production of urease and lipoxygenase enzymes.