Corosolic acid suppresses the expression of inflammatory marker genes in CCL4-induced-hepatotoxic rats

The aim of the study was to asses the anti-inflammatory effects of corosolic acid on the carbon tetrachloride (ccl4) toxicity in rats. liver toxicity was induced by administered ccl4 (single dose (1:1 in liquid paraffin) orally at 1.25 ml/kg. rats were pretreated with cra for 7 days before made ccl4 toxicity at 20 mg/kg bw. the mrna levels of tnf-α,il-6,inos,cox-2 and nf-κb were assayed by reverse transcriptase pcr analysis. the mrna levels of proinflammatory cytokines such as tnf-α,il-6,and the inflammatory markers such as inos,cox-2 and nf-êb were significantly up regulated in ccl4 induced rats and treatment with corosolic acid significantly reduced the expression of the above indicators. our results suggest that the inhibition of tnf-α,il-6,inos,cox-2 and nf-êb by corosolic acid,a potential candidate could possess anti-inflammatory activity besides its hepatoprotective effect in ccl4 liver toxicity in rats.