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Influence of different metals on the activation and inhibition of α-amylase from thermophilic Bacillus firmus KIBGE-IB28
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نویسنده
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zohra r.r. ,ul qader s.a. ,pervez s. ,aman a.
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منبع
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pakistan journal of pharmaceutical sciences - 2016 - دوره : 29 - شماره : 4 - صفحه:1275 -1278
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چکیده
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Thermophilic bacillus firmus kibge-ib28 produced extracellular α-amylase at temperature 70°c. enzyme was partially purified by ammonium sulfate precipitation with 42.80 fold purification and specific activity of 1889.6 u/mg. effects of various metals on enzyme activity were determined and it was found that enzyme activity boosted significantly in presence of ca2+,k2+,ba2+,co2+ and ni2+ whereas zn2+,mg2+,na2+ and cu2+ were found inhibitory at concentration 10mm.
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کلیدواژه
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Activation; Amylase; Bacillus firmus; Inhibition; Metals
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آدرس
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karachi institute of biotechnology and genetic engineering (kibge),university of karachi,karachi, Pakistan, karachi institute of biotechnology and genetic engineering (kibge),university of karachi,karachi, Pakistan, karachi institute of biotechnology and genetic engineering (kibge),university of karachi,karachi, Pakistan, karachi institute of biotechnology and genetic engineering (kibge),university of karachi,karachi, Pakistan
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Authors
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