|
|
|
|
Role of growth media and chemical enhancers in secondary metabolites production from Aspergillus carbonarius (NRL-369) and their pharmaceutical potentials
|
|
|
|
|
|
|
|
نویسنده
|
khan a.a. ,bacha n. ,ahmad b. ,cox r.j. ,bakht j.
|
|
منبع
|
pakistan journal of pharmaceutical sciences - 2016 - دوره : 29 - شماره : 4 - صفحه:1223 -1230
|
|
چکیده
|
The present study investigates the effect of different growth media and chemical enhancer on silent genes in aspergillus carbonarius (nrl-369) for secondary metabolites production and its in vitro biological activities. results revealed that aspergillus carbonarius (nrl-369) grown in czapeak yeast extract broth medium produced more metabolites compared with other media. chemical epigenetic modifiers (suberoyl-anilide hydroxamic acid (saha) and 5-azacytidine (5-aza) at concentration of 15mm were effective for the expression of silent genes resulting in increased secondary metabolites production. secondary metabolites extracted in ethyl acetate and fractionized in n-hexane showed variable degree of growth inhibitions of the tested microorganisms. similarly,these samples were also active against brine shrimps and lemna.
|
|
کلیدواژه
|
Aspergillus carbonarius; Chemical enhancers; Growth media; Pharmaceutical activities; Silient genes
|
|
آدرس
|
institute of integrative biosciences,cecos university of emerging sciences,hayatabad,peshawar,kpk, Pakistan, centre of biotechnology and microbiology,university of peshawar, Pakistan, centre of biotechnology and microbiology,university of peshawar, Pakistan, school of chemistry,university of bristol,cantock's close,bristol, United Kingdom, institute of biotechnology and genetic engineering,university of agriculture peshawar, Pakistan
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Authors
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|