Isolation and identification of antiproliferative compounds from the roots of Tetrastigma hemsleyanum against MDA-MB-435S cell lines

This present study aimed to elucidate antiproliferative activity of four extracts (chcl3,etoac,n-buoh and h2o) and chemical constituents isolated from the most potent extract of tetrastigma hemsleyanum diels et. gilg (tdg) against mda-mb-435s cell lines using the mtt assay at various concentrations in vitro. ten compounds were isolated and identified as (1) β-sitosterol,(2) palmitic acid,(3) protocatechuic acid,(4) salicylic acid,(5) p-hydroxybenzoic acid,(6) resveratrol,(7) trans-4-hydroxycinnamic acid,(8) kaempferol,(9) quercetin,and (10) isoquercitrin. compounds 3,5-7,10 were the first report of isolation from this plant. moreover,antiproliferative activity displayed that the chcl3,h2o extracts and compounds 6,8 exhibited obvious inhibitory effects on mda-mb-435s cell lines with ic50 values 100.28± 2.64,127.48±3.45,92.39±1.68 and 120.30±1.97μg/ml,respectively. thus the obtained results indicate antiproliferative activity of tdg against mda-mb-435s cell lines is ascribable to the most potent chcl3 extract along with active compounds 6 and 8,which could be considered as a potential chemotherapeutic agent in breast cancer.