Effects of ginseng-spikenard heart-nourishing capsule on inactivation of C-type Kv1.4 potassium channel

This research is to explore the effects of traditional chinese medicine ginseng-spikenard heart-nourishing capsule on the inactivation of c-type kv1.4 channels (kv1.4δn) in xenopus laevis oocytes with two-electrode voltageclamp technique. defolliculated oocytes (stage v-vi) were injected with transcribed crnas of ferret kv1.4δn channels. during recording,oocytes were continuously perfused with nd96 solution (control group) and solution prepared from ginseng-spikenard heart-nourishing capsule (experimental group). results found that,at the command potential of +50 mv,the current of experimental group was reduced to 48.33±4.0% of that in control group. the inactivation time constants in control and experimental groups were 2962.56±175.35 ms and 304.13±36.22ms,respectively (p<0.05,n= 7). the recovery time of fkv1.4δn channel after inactivation in control group and experimental groups was 987±68.39 ms and 1734.15±98.45 ms,respectively (p<0.05,n=5). ginseng-spikenard heart-nourishing capsule can inhibit the kv1.4δn channel,which may be one of the mechanisms of underlying antiarrhythmia.