Anti-proliferative and apoptosis inducing potential of hydroalcoholic Achillea wilhelmsii C. Koch extract on human breast adenocarcinoma cell lines MCF-7 and MDA-Mb-468

Achillea wilhelmsii c. koch contains a variety of components such as flavonoid. the previous studies showed that flavonoid has anti-cancer properties. the aim of the present study was to determine the anti-proliferative and apoptosis-inducing potential of hydroalcoholic achillea wilhelmsii c. koch extract (hawe) on mcf-7 and mda-mb-468 human breast carcinoma cell lines. the anti-proliferative activity of hawe was evaluated using mtt,flowcytometry by annexin v/pi double staining,and caspase-3 activity. the results of mtt showed that the ed50 of mcf-7 and mda-mb-468 was 25μg/ml of hawe,48h after treatment. flowcytometry by annexin v/pi showed that hawe induced late apoptosis in mcf-7 and early apoptosis in mda-mb-468. in addition,the caspase-3 colorimetric method showed that caspase-3 increased in the mda-mb-468 after treatment with hawe. this study found that the hydroalcoholic extract of achillea wilhelmsii c. koch induced apoptosis in both the mcf-7 and mda-mb-468 human breast carcinoma cell lines.