Anti-inflammatory effects and mechanisms of Hizikia fusiformis via multicellular signaling pathways in lipopolysaccharide-induced RAW 264.7 cells

In this study we investigated the anti-inflammatory effects and mechanisms of hizikia fusiformis (hf) extracts in lipopolysaccharide (lps)-induced raw 264.7 cells. we extracted hf using solvent and sub-critical water techniques. in results,hf extracts inhibited nitric oxide (no) production in cell-free and lps-stimulated raw 264.7 cells. hf210 (extract prepared with sub critical water at 210°c) was most effective. the hf210 extract dose-dependently inhibited inducible nitric oxide synthase expression (inos) and nuclear factor kappa (nf-κb) p65 translocation from cytosol to the nucleus. furthermore,hf210 extract dose-dependently inhibited the phosphorylation of p38 mitogen-activated protein kinase (p38 mapk),jun n-terminal kinase (jnk),and signal transducers and activators of transcription (stat)-1in lps-induced raw 264.7 cells. thus,our results suggest that anti-inflammatory effects of hf210 extract showed a noticeable distinction by regulation of multiple signaling pathways in lps-induced raw 264.7 cells.