Evaluation of anti-oxidative,antimicrobial and anti-diabetic potential of Adiantum venustum and identification of its phytochemicals through GC-MS

The present study aimed to explore antioxidant,antimicrobial and anti-diabetic properties of the fern adiantum venustum. the tpc (total phenolic content) of methanolic extract of the plant was 247.95±0.0007μg of gallic acid equivalents per gram of dried extract (mg gae/g). the highest tpc was in n-butanolic fraction,which was 981.45±0.1562mg gae/g. hexane fraction showed lowest tpc (256.95±0.0420mg gae/g). ethyl acetate fraction exhibited highest total flavonoid content (tfc),i.e.,62.0±0.050mg of rutin equivalents per gram of sample. dpph (1,1- diphenyl-2- picryl hydrazyl) radical scavenging activity of the plant was significant. n-butanolic fraction was most potent with ic50 being 1.06mg/ml. the ic50 of methanolic extract was 1.50mg/ml,that of aqueous fraction was 2.51 mg/ml,and that of chloroform fraction was 2.65mg/ml. antibacterial potential of the fern was tested against two grampositive bacterial strains,pseudomonas aeruginosa and escherichia coli and two gram-negative bacterial strains,bacillus subtilis and staphylococcus aureus. n-butanolic fraction showed highest zone of inhibition (zoi,25.13±1.237 mm) against p. aeruginosa. ethyl acetate fraction was most active (zoi,15.75±1.060 mm) against s. aureus. against e. coli,aqueous and n-butanolic fractions were most active (zoi,14.75±0.353 and 14.50±0.707mm,respectively). minimum inhibitory concentration (mic) of methanolic extract against b. subtilis was as low as 1.98mg/ml. the hexane fraction was least toxic against all the fractions. the plant displayed significant alpha-amylase inhibitory activity. chloroform fraction was most effective with lowest ic50 (1.10mg/ml) followed by ethyl acetate fraction (1.92mg/ml). a. venustum is rich in phenolics and has substantial antioxidant,antimicrobial and alpha-amylase inhibitory potential.