To estimate effective antiamyloidogenic property of melatonin and fisetin and their actions to destabilize amyloid fibrils

Aggregating of amylin as pancreatic deposition is connected with pancreas degeneration in type 2 diabetes mellitus. suppression of the amylin accumulation and so instability of the pre-formed pancreatic β-amyloid,may be attractive curative goal for mediation of diabetes mellitus. fluorimetric assay by thioflavin-t was utilized for investigating the properties of melatonin and fisetin on the generation and instability of β-amyloid near to physiological conditions. the results showed that after 168 hours incubation by shaker incubator in 37oc,melatonin at 10μm and 40 μm repressed amylin amyloid formation by 20.1% and 27.5% respectively (p<0.05) and the similar values of fisetin inhibited the formation of β-sheet structure by 16.5% and 23.2% respectively (p<0.05).the obtained data also confirmed that amyloidal sheet opening was induced by melatonin and fisetin significantly (p<0.05). it may be concluded that islet amyloid cytotoxicity to β-cells may be reduced by melatonin and fisetin,and they should be important constituents of new drugs for diabetes mellitus treatment.