HPLC method development and validation for the determination of Cefaclor in human plasma

Cefaclor was analyzed in the human plasma by developing a simple,precise and accurate assay method which was then validated for its accuracy,specificity and precision. the mobile phase comprised of a mixture of sodium 1- pentanesulfonate,water,triethylamine and methanol. phosphoric acid was used to adjust the ph to 2.5±0.1. the flow rate was maintained at 1.5ml/min and the wavelength was set at 265 nm. a c-18 hplc,column 5um particle size,l x 1.d. 25cm x 4.6mm (supelcosil) was utilized for chromatographic separation. the retention time of cefaclor was found to be 17min. this method was validated for selectivity,accuracy,precision,repeatability,reproducibility,recovery,linearity,and stability. calibration curves were found linear were in the range of 0.39μg/ml to50μg/mland the coefficient of correlation (r2) was found to be 0.999. hence,this method has been found useful for the determination of cefaclor in plasma.