GC-MS-based metabolomics analysis of transgenic rice with human serum albumin

This study was to analyze the difference of the metabolite profiles between non-transgenic (tp309-8) and human serum albumin (hsa) transgenic rice (tp309-hsa-8,tp309-hsa-9,corresponding to 8th and 9th generation) by gas chromatography-mass spectrometry followed by multivariate analyses methods including principal component analysis (pca),partial least squares-discriminant analysis (pls-da) and hierarchical cluster analysis (hca). as a result,12 differential metabolites were identified between tp309-hsa-8 and tp309-8,of which 6 were known compounds (trehalose,citric acid,valine,glycine,asparagine and pantothenic acid) and they were enriched in starch and sucrose metabolism,carbon fixation pathways in prokaryotes,valine,leucine and isoleucine degradation and biosynthesis,glycine,serine and threonine metabolism,and antidyslipidemic agents pathways,respectively. there were 4 different compounds between tp309-hsa-8 and tp309-hsa-9,including known compounds [asparagine and oleic acid (c18:1)]. however,no pathways were enriched for them. our findings preliminarily reveal transgenic hsa may be beneficial for rice growth and providing more essential amino acid for human beings by altering the metabolite profiles. © 2017,pakistan botanical society. all rights reserved.