Optimizing the rapid technique for propagation of Cerasus campanulata by tissue culture

The initiation medium for cerasus campanulata was 1/2ms+1.0 mg l-1 ba+0.1 mg l-1 naa +30 g l-1 sugar,and the average initiation rate was 94.4%. the proliferation medium composed of ms+0.3 mg l-1 ba+0.2 mg l-1 naa+30 g l-1 sugar provided proliferation rate 3.6 and average shoot length 2.3 cm. the rooting medium composed of 1/2ms+0.6 mg l-1 iba+20 g l-1 sugar provided rooting rate 85.6%,average root number per individual 5.9 roots,and average root length 2.7 cm. the dishwashing detergent instead of tween-20 as the spreader reduced the contamination rate for the explant disinfection. the red-core soil adopted to be the plantlet transfer medium provided 91.3% survival rate. the light media lz and sk were not eligible to be the transfer medium for the species. © 2016,pakistan botanical society. all rights reserved.