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The differential gene expression of key enzyme in the gibberellin pathway in the potato (Solanum tuberosum) mutant M4P-9
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نویسنده
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shi j.-b. ,ye g.-j. ,yang y.-z. ,wang f. ,zhou y. ,sun h.-h. ,wang j.
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منبع
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pakistan journal of botany - 2016 - دوره : 48 - شماره : 3 - صفحه:1107 -1117
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چکیده
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In the present study,the expression patterns of the key genes in the gibberellin synthesis pathway in the potato dwarf mutant m4p-9 were detected using quantitative real-time pcr. using actin as an internal control,cps1,ks,ko,ga20ox1,and ga2ox1,genes for key gibberellin synthesis enzymes,were evaluated,along with a gibberellin receptor gene. the standard curves were obtained from dilutions of pcr product; the correlation coefficient for actin was 0.995,and those for the target genes varied from 0.994 to 1.000. the expression patterns of gibberellin pathway genes in different growth stages and tissues were calculated according to the method of pfaffl. these genes showed expression patterns that varied based on growth stage and tissue type. the higher expression levels of cps1 and ga2ox1 in roots,the lower expression levels of ga20ox1 in roots during tuber formation stage; as well as the increased expression of ga20ox1 and ga2ox1 genes in stems during the tuber formation stage,likely play key roles in the plant height phenotype in m4p-9 mutant materials. this article provides a basis for researching the mechanism of gibberellin synthesis in potato. © 2016,pakistan botanical society. all rights reserved.
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کلیدواژه
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Gene expression; Gibberellin pathway; Solanumtuberosum
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آدرس
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qinghai university,xining, China, qinghai university,xining,qinghai,china,qinghai academy of agricultural and forestry sciences,xining,qinghai,china,key lab of qinghai-tibet plateau biotechnology of ministry of education,xining,qinghai,china,key lab of germplasm innovation and utilization of plateau crop of qinghai province,xining,qinghai,china,state key lab of three river sources ecology and plateau farming and animal husbandry,xining, China, qinghai university,xining,qinghai,china,qinghai academy of agricultural and forestry sciences,xining,qinghai,china,key lab of qinghai-tibet plateau biotechnology of ministry of education,xining,qinghai,china,key lab of germplasm innovation and utilization of plateau crop of qinghai province,xining,qinghai,china,state key lab of three river sources ecology and plateau farming and animal husbandry,xining, China, qinghai university,xining,qinghai,china,qinghai academy of agricultural and forestry sciences,xining,qinghai,china,key lab of qinghai-tibet plateau biotechnology of ministry of education,xining,qinghai,china,key lab of germplasm innovation and utilization of plateau crop of qinghai province,xining,qinghai,china,state key lab of three river sources ecology and plateau farming and animal husbandry,xining, China, qinghai university,xining,qinghai,china,qinghai academy of agricultural and forestry sciences,xining,qinghai,china,key lab of qinghai-tibet plateau biotechnology of ministry of education,xining,qinghai,china,key lab of germplasm innovation and utilization of plateau crop of qinghai province,xining,qinghai,china,state key lab of three river sources ecology and plateau farming and animal husbandry,xining, China, qinghai university,xining,qinghai,china,qinghai academy of agricultural and forestry sciences,xining,qinghai,china,key lab of qinghai-tibet plateau biotechnology of ministry of education,xining,qinghai,china,key lab of germplasm innovation and utilization of plateau crop of qinghai province,xining,qinghai,china,state key lab of three river sources ecology and plateau farming and animal husbandry,xining, China, qinghai university,xining,qinghai,china,qinghai academy of agricultural and forestry sciences,xining,qinghai,china,key lab of qinghai-tibet plateau biotechnology of ministry of education,xining,qinghai,china,key lab of germplasm innovation and utilization of plateau crop of qinghai province,xining,qinghai,china,state key lab of three river sources ecology and plateau farming and animal husbandry,xining, China
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