Conventional breeding enhances genetic diversity in Pisum sativum (L.) detected through SDS-PAGE

In the present study,twenty homozygous segregante of edible pea were developed through conventional breeding (2004-2015). the seed storage proteins were extracted from the seeds of each line and run through slab-vertical gel electrophoresis. after staining and de-staining the electrophoregram was divided into two zones (r-i and r-ii). the r-1 was consists of five bands (b-1,2,3,4 & 5) ranging from 180kda to 34kda,which calculated < 1% genetic diversity (gd). out of which b-1,2 and 3 were polymorphic,while b-4 and b-5 were monomorphic. due to strong genetic association and low level of gd,the r-i divided the total germplasm into two clusters. similarly,r-ii was comprised of nine bands ranging from 33kda -10kda. all the loci were polymorphic and showed >39% genetic diversity. the r-ii divided the total lines into four clusters. in cluster plating,43.74% lines were grouped in c-03,30.43% in c-01,13.39% in c-04 and 8.69% in c-02. variation in bands profile and multiple clustering authenticates that conventional plant breeding enhances the level of genetic variation in seed storage protein profile in pea germplasm detected through sds-page. © 2016,pakistan botanical society. all rights reserved.