Chemical composition of essential oil from in vitro grown Peperomia obtusifolia through GC-MS

Apical meristems and nodal plant parts were used for mass propagation of peperomia obtusifolia. different concentrations of bap (6-benzylaminopurine),tdz (thidiazuron) and kin (kinetin) were used in ms medium. the highest shoot proliferation and multiplication formation occurred in the ms basal medium containing 1.0 mg/ml bap. maximum number of shoots and shoot lengths were 9.80 ±0.50 and 35.40 ± 2.92 cm respectively. rooting response was the best in ms basal medium fortified with1.0 mg/ml bap plus 0.5 mg/ml naa (α-naphthalene acetic acid). for acclimatization,the rooted plantlets were transferred to the greenhouse. the volatile oil of this in vitro grown p. obtusifolia was extracted by hydro-distillation and investigated by gas chromatography/mass spectrometry (gc-ms). the chromatographic analysis of oil showed 35 constituents of which,16 volatile compounds contributing 65.0% of the total oil constituents could be identified. the major components identified in this oil were sesquiterpenes such as caryophyllene (17.17%),apiol (16.65%),α-cardinol (2.12%) and α-caryophyllene (1.90%). the monoterpenes such as r-α-pinene (1.00%),camphene (0.84%),borneal (0.32%) and limonene (0.25%) were also identified. the tau.-muurolol (0.68%),τ- elemene (0.63%),copaene (0.43%) and tau.-cadinol (0.27%) were present comparatively in minor percentages. the caryophyllene oxide (2.95%) was oxygenated sesquiterpene,3,5-dimethoxy-4-hydroxycinnamic acid (0.53%) and asarone (0.41%) were phenyl-propanoids.