Expression analysis of Fusarium wilt resistance gene in melon by real-time quantitative PCR

Melon actin gene was used as a reference gene,to explore the gene expression profiles of the fom-2 gene in roots,stems,and leaves of melon mr-1 under induction by fusarium oxysporum f. sp. melonis. monitoring using real-time quantitative pcr showed similar accumulation patterns of fom-2 in roots,stems,and leaves over the observation period of 1 to 11 days; the expression level in stems was the highest. the expression of the fom-2 gene was strengthened by the prolongation of induction time. in stems,the expression of fom-2 was 5.737 times higher than in the control at three days; in roots,expression of fom-2 was 5.617 times higher than in the control at five days. similarly,the expression of fom-2 in leaves obviously increased. it was 4.441 times higher than in the control at 5 days. the expression of fom-2 was non-tissue specific,up-regulated under induction by fusarium,and related to early resistance to fusarium wilt.