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Influencing factors of embryo rescue in seedless grape
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نویسنده
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guo x. ,chen w. ,guo y. ,liu z. ,lin h. ,tang j. ,li k. ,zhao y.
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منبع
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pakistan journal of botany - 2015 - دوره : 47 - شماره : 2 - صفحه:669 -673
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چکیده
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In this study,we investigated the impact of inoculating stage,medium type and concentration of plant growth regulators on embryo rescue effectiveness by l 25(5)6 orthogonal design using selfed ovules of ‘venus seedless’ as the testing material. the main results were as follows. the most important factor influencing ovule germination was inoculating stage. ovule germinating rate gradually increased as inoculating being postponed. the highest germinating rate appeared when inoculation was done 55d after flowering. other influencing factors were iba concentration,exogenous amino acid,6-ba concentration,ga3 concentration and medium type in descending order. the best embryo rescue result was based on nitsch medium including 1.0 mg/l iba,0.1 mg/l ga3,0.7 mg /l 6-ba and 2.0 mmol/l glutamine using ovules inoculated 55d after flowering. the highest germinating rate reached 41.25%,and a batch of seedlings was also obtained. © 2015,pakistan botanical society. all rights reserved.
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کلیدواژه
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Embryo rescue; Influencing factors; Venus seedless grape
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آدرس
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college of horticulture,shenyang agricultural university, China, college of horticulture,shenyang agricultural university, China, college of horticulture,shenyang agricultural university, China, college of horticulture,shenyang agricultural university, China, college of horticulture,shenyang agricultural university, China, college of horticulture,shenyang agricultural university, China, college of horticulture,shenyang agricultural university, China, college of horticulture,shenyang agricultural university, China
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Authors
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