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A simple method for microtuber production in Dioscorea opposita using single nodal segments
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نویسنده
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li m. ,li j. ,wang y. ,liu w. ,guo x. ,li s. ,han l. ,song z. ,zhao x. ,yang q.
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منبع
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pakistan journal of botany - 2015 - دوره : 47 - شماره : 2 - صفحه:665 -668
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چکیده
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Dioscorea opposita thunb. (chinese yam) is an important tuber crop in east asia because of its dual benefits edible and medicinal properties. microtubers may provide a feasible alternative to in-vitro-grown plantlets as a means of micropropagation and a way to exchange healthy planting material. in this study,we have developed a simplified culture method for in vitro production of microtubers from d. opposita cv. ‘tiegun’. in this method,microtubers formed in 98% of the internodes of single nodal segments after four weeks of dark-incubation when cultured in ms medium supplemented with 60 g sucrose l-1 with shaking. anatomical observations strongly supported the process of tuberization. we also found that 66% of the microtubers produced in vitro sprouted two months after transfer to vermiculite. the protocol presented here provides a simple model for studying the physiological,biochemical,and molecular mechanisms of tuberization in d. opposita,and shows good potential for large-scale production of microtubers as well. © 2015,pakistan botanical society. all rights reserved.
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کلیدواژه
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Dioscorea opposita; Liquid medium; Microtuber; Shaking culture; Single nodal segments
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آدرس
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college of life sciences,henan normal university,xinxiang,china,engineering technology research center of nursing and utilization of genuine chinese crude drugs,university of henan province,xinxiang,china,engineering laboratory of green medicinal material biotechnology,xinxiang, China, college of life sciences,henan normal university,xinxiang,china,engineering technology research center of nursing and utilization of genuine chinese crude drugs,university of henan province,xinxiang,china,engineering laboratory of green medicinal material biotechnology,xinxiang, China, college of life sciences,henan normal university, China, college of life sciences,henan normal university, China, college of life sciences,henan normal university, China, college of life sciences,henan normal university, China, college of life sciences,henan normal university, China, college of life sciences,henan normal university, China, college of life sciences,henan normal university,xinxiang,china,engineering technology research center of nursing and utilization of genuine chinese crude drugs,university of henan province,xinxiang,china,engineering laboratory of green medicinal material biotechnology,xinxiang, China, college of life sciences,henan normal university,xinxiang,china,key laboratory for microorganisms and functional molecules,university of henan province, China
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Authors
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