Optimization of fermentation medium for L-lysine production by Corynebacterium glutamicum

Corynebacterium glutamicum is an industrially important microorganism used for amino acids production. the present work describes the production of l-lysine from corynebacterium glutamicum iib-c9 through submerged fermentation process. fifteen fermentation media were screened in this study for l-lysine production. out of these fifteen media,fm:13 was found a best one for maximum production of l-lysine i,e.,2.5g/l. the medium contained glucose,ammonium sulphate,dipotassium hydrogen phosphate,potassium dihydrogen phosphate,calcium carbonate,thiamine hcl,biotin,manganese chloride tetrahydrate and casamino acids. this medium was further supplemented with different nitrogen and carbon sources to increase the l-lysine production. glucose,as best source of carbon and ammonium sulphate as a best nitrogen source were optimized. finally 3.5 g/l of l-lysine was produced after 72 hrs incubation in fm:13 medium supplemented with 7% glucose and 2.5% ammonium sulphate © 2015,pakistan botanical society. all rights reserved.