Protein profiles of phytoagglutinins from indigenous species of euphorbiaceae,Leguminosae and moraceae from Pakistan

The present study was aimed at the identification of plant lectins from indigenous plants belonging to leguminosae (albizia lebbeck,cassia fistula,pongamia glabra,acacia catechu),moraceae (morus nigra,m. alba,ficus indica,ficus sp.,broussonetia papyrifera) and euphorbiaceae (euphorbia helioscopia,jatropha hastata,putranjiva roxburgii). soluble proteins were selectively isolated in 0.15 m nacl and the presence of agglutinin activities against erythrocytes of blood groups a,b,ab and o was monitored in different organs of the plants. the protein content of the extract was determined by pyrogallol red method. an sds gel electrophoresis protocol was developed to separate proteins in crude extracts as well as fractions obtained by fast protein liquid chromatography of some selected plants. the gel electrophoresis was carried out using a bio-rad mini gel assembly. several distinct protein profiles were observed both for the crude protein extracts and samples fractionated by fast protein liquid chromatography (fplc). these proteins corresponded with strong agglutinin activities particularly in albizia lebbeck (black siris),cassia fistula (amaltas),pongamia glabra (sukh chaen),aacacia catechu (katha) morus nigra (siah toot),m. alba (bedana) and putranjiva roxburghii (patanjan). © 2015,pakistan botanical society. all rights reserved.