Effect of endophytic pseudomonas aeruginosa and trichoderma harzianum on soil-borne diseases,mycorrhizae and induction of systemic resistance in okra grown in soil amended with Vernonia anthelmintica (L.) seed’s powder

Biostimulants are used in agricultural practices for plant growth improvement. these fertilizers improve microbial activity and cause a negative impact on soil-borne pathogens. in recent years,stimulating plant’s natural defense is considered as most promising alternative strategy for crop productivity. the present study was carried out to examine the effect of endophytic pseudomonas aeruginosa and trichoderma harzianum in soil amendment with vernonia anthelmintica seed’s powder,on root rotting fungi,plant growth,mycorrhizal population around roots,phosphorous uptake and stimulation of plant defense markers like poylphenol and antioxidant status in okra. combine application of vernonia with pseudomonas aeruginosa and trichoderma harzianum significantly (p<0.05) suppressed rhizoctonia solani and fusarium oxysporum with complete reduction of macrophomina phaseolina and fusarium solani. pseudomonas aeruginosa and t. harzianum alone or in vernonia amended soil significantly reduced nematode’s galls on roots. organic amendment also improved plant resistance against root diseases as evident from enhanced dpph radical scavenging capacity and polyphenol content in treated plants as compare to control. va mycorrhizal spores were found significantly (p<0.05) higher in number around roots received pseudomonas aeruginosa or t. harzianum alone or in vernonia amended soil. whereas,higher concentrations of phosphorus in okra shoots were found in plants received biocontrol agents in amended soil. mixed application of pgpr and t.harzianum in amended soil produced tallest plants than other treatments. soil amendment with vernonia seed’s powder alone or with biocontrol agents offer a nonchemical means of plant disease control. © 2015,pakistan botanical society. all rights reserved.