Tissue culture studies in tomato (Lycopersicon Esculentum) var. Moneymaker

A protocol was developed for callus induction and regeneration in tomato (lycopersicon esculentum) var. moneymaker. hypocotyl and leaf disc explants of tomato were used as a starting material for callus induction. explants were cultured on ms medium having different concentrations of hormones. maximum callogenesis from hypocotyls was obtained on ms medium supplemented with iaa (2 mg/l),naa (2 mg/l),bap (5 mg/l) and kin (4 mg/l). it was 65.2% for hypocotyls. for leaf discs maximum callogenesis was achieved on ms medium supplemented with iaa (2 mg/l),naa (2 mg/l),bap (2 mg/l) and kin (4 mg/l). it was 81.3% for leaf discs. calli were cultured on ms medium having concentrations of zeatin (1 mg/l) and iaa (1 mg/l) for regeneration. they showed maximum regeneration of 69.2% from hypocotyls. minimum regeneration of 2.8% was obtained on ms medium supplemented with iaa (2 mg/l),naa (2 mg/l),bap (2 mg/l) and kin (4 mg/l). tomato shoots were shifted to 1/2 ms medium containing iba (0.1 mg/l) and bap (0.0025 mg/l) for rooting and all responded positively to rooting.