Determination of taxonomic status of Chinese species of the genus Clematis by using high performance liquid chromatography-mass spectrometry (HPLC-MS) technique

A comparative taxonomic study using chemometric and numerical taxonomic approaches on 15 populations of 12 species of major taxa of the genus clematis,belonging to sections,rectae,clematis,meclatis,tubulosae and viorna were analyzed by hplc coupled with diode array detector and esi-ms. the chemodiversity profile of saponins proved to be useful taxonomic markers for the genus and results are presented in phenograms. the compound 'huzhangoside d' was the most abundant in analyzed species of the genus. numerical taxonomic study was also conducted based on phylogenetically informative characters which corroborated with chemical fingerprinting findings. the significance of chemical markers in taxonomic study as well as their correlation between morphology and chemical compound profile is also debated with its significant role in botanic drugs identification.