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Proteomics approach to identify differentially expressed proteins induced by iron deficiency in roots of Malus
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نویسنده
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wang j.-y. ,ruan s.-l. ,wu w.-h. ,xu x.-f. ,wang y. ,han z.-h.
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منبع
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pakistan journal of botany - 2010 - دوره : 42 - شماره : 5 - صفحه:3055 -3064
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چکیده
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Malus xiaojinensis (malus xiaojinensis cheng et. jiang) is a fe-efficient apple. we report for the first time a systematic proteomic approach to iron stress-responsive proteins in roots of malus. plantlets of malus were cultured in hydroponic system without fe element for 3 d. after then,total proteins were extracted from roots of the treated plantlets and separated by two-dimensional gel electrophoresis (2-de). more than 700 protein spots were reproducibly detected. the matrixassisted laser desorption/ionisation-time of flight and peptide mass fingerprinting (pmf) analysis and database searching helped us identify 12 up-regulated protein spots representing 10 different proteins. two spots were putative pyridoxine biosynthesis protein isoform b,2 were identified as sadenosylmethionine synthetase (sams) and other 8 as fructokinase (fk),isocitrate dehydrogenase (nadp),monodehydroascorbate reductase (madr),ap003246 nid,actin,hypothetical protein,probable catechol o-methyltransferase (comt) and vacuolar h+-atpase (v-atpase) catalytic subunit,respectively. these proteins were involved in regulation of carbohydrate and energy metabolism,stress-defense response,and membrane transport. something relevant to fe deficiency stress was discussed.
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آدرس
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key laboratory of beijing municipality of stress physiology and molecular biology for fruit trees,institute for horticultural plants,china agricultural university,beijing,100193,china,college of plant science,jilin university,changchun, China, institute of biotechnology,hangzhou academy of agricultural sciences,hangzhou, China, state key laboratory of plant physiology and biochemistry,college of biological,china agricultural university,national plant gene research center,beijing, China, key laboratory of beijing municipality of stress physiology and molecular biology for fruit trees,institute for horticultural plants,china agricultural university,beijing, China, key laboratory of beijing municipality of stress physiology and molecular biology for fruit trees,institute for horticultural plants,china agricultural university,beijing, China, key laboratory of beijing municipality of stress physiology and molecular biology for fruit trees,institute for horticultural plants,china agricultural university,beijing, China
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Authors
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