Novel protocol for albumin and globulin detection in Pisum sativum genotypes using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)

In the present study a novel protocol for the removal of albumin and globulin in 10 genotypes of pisum sativum were developed by using osborn solubility class protein extraction buffer (10mm tris-hcl buffer,ph 6.8,0.5 m nacl). one major allocation of protein band and one minor protein band corresponding to 10 kda and 22 kda,respectively,was detected in sds-page (untreated cases). there is a need to further improve this method so that the minor groups of albumin and globulin could also be investigated further to characterize through 2d gel electrophoresis.