In vitro germination of hybrid embryos from interploidal crosses of citrus

Interploid crosses between feutrell's early (2x),kinnow mandarin (2x),succari sweet orange (2x) and kinnow (4x) were made for the production of triploid hybrids. maximum fruit set (100%) was observed between crosses of kinnow (2x) x kinnow (4x) and maximum june drop (95.5%) was noted when diploid succari as female parent was crossed with tetraploid kinnow. the hybrid fruits harvested after 12-14 weeks or 7 months after pollination produced many underdeveloped and few developed seeds; however,tetraploid kinnow as seed parent yielded more developed seeds per fruit. immature embryos of seeds harvested 12-14 weeks after pollination were cultured in vitro on ms medium supplemented with adenine sulphate (0.25 g l -1),malt extract (0.5 g l -1) and sucrose (50 g l -1). maximum germination (58.6%) of hybrid embryos from developed seeds was obtained in 4x x 2x cross of kinnow strains.