Process optimization for a potent wild and mutant strain of Aspergillus niger for biosynthesis of amyloglucosidsae

The present study is concerned with the selection of a potent strain of aspergillus niger and optimization of the cultural conditions for the biosynthesis of amyloglucosidase. the cultural conditions were optimized for the enzyme production. twenty percent (50/250ml flask) was found to be optimum volume of the medium. optimum temperature was 30 oc after 72 h of incubation,with the initial ph of the medium 5.0. 2% starch with 1% glucose as an additional carbon source gave maximum amyloglucosidase production addition of 0.3% ammonium sulphate in the fermentation medium increased the enzyme production while 2% spore inoculum showed best amyloglucosidase production.