Partial characterization of bacteriocin like inhibitory substance from Bacillus subtilis BS15,a local soil isolate

The aim of this study was to investigate the production of bacteriocin/bacteriocin-like inhibitory substances (blis) from bacillus subtilis bs15,isolated from soil. the inhibitory substance was partially purified and characterized as blis with a molecular-weight of 3-5 kda,as determined by sds-page. its production was observed during the late exponentialphase or at the beginning of stationary-phase. it retained its activity up to 80°c and over a wide range of ph i.e.,3-9. it was found active against several clinically important bacterial species such as listeria monocytogenes,staphylococcus aureus,bacillus cereus,salmonella typhi and also against the food-spoilage causing microbes,and may be considered as future food preservative.