In vitro mutagenesis in banana and variant screening through ISSR

An efficient culture medium for in vitro clonal propagation of banana cultivars was established. meristamtic tips of basrai and gctcv-215 were irradiated with different doses of gamma rays viz.,0,10,20,30 and 40gy through cobalt 60 source. eleven random issr primers were used to study genetic variation among the radiated population. issr primers produced 37 bands in all the clones studied. two monomorphic and 35 polymorphic bands were observed during the study. the number of fragments produced by the primers ranged from 2-5,with an average of 3.45 fragments. fragments size ranged from 198bp-1.842kb. genetically most similar genotypes were g-215mp3 and g-215mp4 (89.8%) while most dissimilar genotypes were basrai and g-215mp2 (38.5%). on the basis of similarity matrix clones could be divided into three clusters and two groups.