Callus induction and plant regeneration from leaf explant of Cornukaempferia aurantiflora mood & larsen

Young leaves of cornukaempferia aurantiflora mood & larsen were used for the initiation of callus growth on murashige skoog medium supplemented with 3% sucrose and various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-d) in the light. the highest number of callus forming,percentage of callus formation and average weight of callus were obtained from young leaves cultured on the medium supplemented with 2.0 mg/l 2,4-d. shoots were successfully regenerated on the medium with concentrations of 2,4-d and ba added.