Assessment of relocation of Trigonella cylindraceae L. and T. polyceratia (L.) trautv. to genus Medicago as inferred by RAPD and RFLP analyses

The taxonomic relocation of both trigonella cylindraceae and t. polyceratia either to trigonella or medicago was assessed based on two molecular markers,rapd and rflp. through rapd analysis,109 bands were generated by ten random primers with an average 11 products/primer. the maximum number of rapds (76 bands) associated with the highest percentage of polymorphism (43.3%) was recorded for t. polyceratia,while the minimum was observed in t. foenum-graecum (63 bands and 32.3%). rflp analysis of 18s rdna was performed by using five restriction endonucleases (ecori,hindiii,bamhi,hinfi and tagi). the restriction endonuclease hinfi and tagi produced two and three fragments respectively,and no variation was observed in these sites among the studied species. however,another three restriction endonuclease (ecori,hindiii & bamhi) were undigested. the present study suggested to reallocate of both t. cylindraceae and t. polyceratia to medicago and certified the value of rapd analysis for the taxonomic intention.