Plant regeneration from irradiated embryogenic callus of sugarcane

Three sugarcane clones viz.,nia-98,nia-0819 and bl4 were used for induction of genetic variability through in-vitro mutagenesis. apical meristametic region was used for callus induction on 4mg/l 2,4-d. actively growing callus was treated with four different doses of gamma rays (10gy,20gy,30gy and 40gy). maximum callus,proliferation and plantlets regeneration growth was observed in control and minimum at 40gy. maximum chlorophyll mutation frequency was recorded in 30 and 40 gy. maximum number of roots was observed in control followed by 10gy. the maximum root length (10.3cm) was obtained in control at 2 mg/l iba. the maximum number of tiller in the irradiated population was observed in 10gy. the treatments 30gy and 40gy exhibited negative impact on the tillering potential of the plant.