Depletion of Y associated proteins by RNAi down-regulates the Y RNA in Caenorhabditis elegans

Short non-coding rnas (ncrnas) perform their cellular functions in ribonucleoprotein (rnp) complexes,which are also essential for maintaining the stability of the ncrnas. depletion of individual protein components of y ribonucleoprotein (y rnp) particles by rna interference (rnai) in caenorhabditis elegans produced specific phenotypes. northern blot also confirmed the depletion of targeted y rnp protein component. the depletion of specific y rnp affected expression levels of the y rna. depletion of candidate associated proteins may constitute an alternative strategy when investigating ncrna-protein interactions and ncrna functions. depletion of both the protein components (ro60 and la) of y rnp complex indicated that these associated proteins are essential for the stability of the y rnas in c. elegans. rnai technique when employed directly against y rna did not produced any phenotype and northern blot showed no effect on the levels of y rna indicating that rnai is not effective against y rna in c. elegans.