A primary study of high performance transgenic rice through maize Ubi-1 promoter fusing selective maker gene

Based on the expression vector pbi121,we successfully constructed a plant overexpression vector of hspa4 gene fusing with selective maker gene (hygromycin-resistance gene) driven by the ubi-1 promoter (pbi121-ubi-hpt-hspa4,p121uhh). the plant expression vectors p121uhh and pcambia1301-ubi-hspa4 (p1301uh) were transformed into the rice callus,mediated by agrobacterium tumefaciens. we screened 17 p121uhh-positive transgenic plants and 15 p1301uh-positive transgenic plants by the hygromycin-resistance gene. the pick-up rate of the resistance callus was 51.7% and 42.5%,respectively,and the rate of regeneration for the resistance callus was 51.2% and 49.1%,respectively. the result of polymerase chain reaction (pcr) identification indicated that the pick-up rate of positive transgenic plants was 51.7% and 42.5% and the total transformation efficiency was 16.5% and 6.2%,and the former was 2.66 times of the later. the results of the experiment indicate that the possibility of the appearance of false positive results in the fusing of a plant overexpression vector with a selective maker gene is much less.