Optimization of the culture conditions in the rice (Oryza sativa cv. Basmati-370)

The objective of the present study was to develop an efficient callus induction protocol for a fine rice (oryza sativa l.) variety basmati-370. mature grains of this variety were used for callus induction. the chu's n6 medium was used as basal medium for callus induction and was supplemented with sucrose,myo-inositol,casein,agar (4 g l-1,5 g l-1,6 g l-1) and growth hormone 2,4-dichlorophenoxy acetic acid in different concentrations of 2ppm and 2.5ppm. the genotype responded well for all the three concentrations of agar (4 g l-1,5 g l-1,6 g l-1) and two concentrations of 2,4-d (2ppm,2.5ppm),however,the combination of 4 g l-1 agar with 2.5ppm 2,4-d gave excellent results with maximum callus induction of 93.42% followed by 4 g l-1 agar with 2ppm 2,4-d and 5 g l-1 with 2.5ppm 2,4-d which also gave better results with the observed callus induction of 92.75% and 91.11%,respectively.