Detection of mycotoxins in maize seed samples

Present results describe the detection of mycotoxins in samples of maize seed collected from different localities of pakistan viz.,karachi,hyderabad,nawabshah,sukhur,lahore,quetta,peshawar and islamabad. seed samples were analyzed quantitavely by competitive direct enzyme linked immunosorbent assay technique (cd-elisa). out of fifty nine samples tested,50 samples were found to be contaminated with aflatoxin whereas 43 samples contained zearalenone and ochratoxin was detected from 4 seed samples. the amount of aflatoxin was detected in high quantity from five samples,while zearalenone was also detected in highest quantity from six samples and ochratoxin detected only in four samples in low quantity.